Endocrine Abstracts

The measurement of 25OH vitamin D has tremendously evolved since the first competitive protein-binding assay. Amongst the different techniques that are now routinely employed, ELISA still represents a common tool to quantify the level of 25OH Vitamin D in individuals. Several 25OH Vitamin D ELISA assays have been developed and commercialized in the last 3 years. They all differ by the antibody used and by the technology that is applied to release 25OH Vitamin D from its bindin...

The objective of this project was to develop and characterise a simple immunoassay for the quantification of free 25-hydroxyvitamin D (25OH Vit D). Recent studies suggest that the concentration and genotype of vitamin D binding protein (DBP) are important factors that determine the bioavailability of 25OH Vit D in blood. It has been suggested that measurement of free, non-protein bound 25OH Vit D in serum, may provide more relevant diagnostic information than total 25OH Vit D,...